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Invited speakersPr. Isabelle Marcotte, University of Québec Montréal (Canada)
Professor Isabelle Marcotte obtained a PhD in chemistry in 2003 at Université Laval, under the supervision of Prof. Michèle Auger. During her PhD, she focused on the study of membrane systems by solid-state NMR. She was especially interested in the interaction of peptides with phospholipid model membranes. She then moved to Zurich (2003-2006) to pursue postdoctoral research under the guidance of Prof. Beat Meier at the ETH, where she gained experience in the solid-state NMR study of complex proteins such as spider silk. In 2006, she was recruited by the Université du Québec à Montréal, and moved back to her hometown, where she established her laboratory dedicated to the study of complex biological systems by solid-state NMR. With her team, they develop model membranes and study protein fibers such as mussel byssus, also known as sea silk. They also develop strategies to investigate intact cells by solid-state NMR. Her lab is particularly interested in looking at interactions of antimicrobial peptides with intact bacteria, and characterizing microalgae for environment applications. In 2014, she obtained the Young Researcher Award of the Université du Québec à Montréal’s Faculty of Sciences. She is Full Professor at the Department of Chemistry since 2015, and Associate Dean of Research of the Faculty of Sciences since 2017. Pr. Arthur Edison, University of Georgia (USA)
Art Edison is a Georgia Research Alliance Eminent Scholar and Professor of Genetics and Biochemistry and a member of the Complex Carbohydrate Research Center and Institute of Bioinformatics at the University of Georgia. His undergraduate degree was in Chemistry from the University of Utah where he worked with David Grant and Bill Epstein to use NMR to characterize monoterpenes from sagebrush. He received his Ph.D. in biophysics from the University of Wisconsin-Madison, where he developed and applied NMR experimental and theoretical methods for protein structural studies under the supervision of John Markley and Frank Weinhold. He was a Jane Coffin Childs postdoctoral fellow with Tony Stretton at UW-Madison and studied neuropeptides in the parasitic nematode Ascaris suum. He joined the faculty at the University of Florida and the National High Magnetic Field Laboratory in 1996. He advanced from Assistant to Full Professor in the UF Department of Biochemistry & Molecular Biology. Prof. Edison was the founding PI and Director of the NIH-funded Southeast Center for Integrated Metabolomics, and his research focuses on the role of small molecules in biology and disease. In 2015, Edison moved to the University of Georgia where he directs the CCRC NMR facility, which supports research in both metabolomics and structural biology. Edison’s research group collaborates on several metabolomics projects from microbes to humans. Most recently, Prof. Edison and colleagues at the University of Connecticut and the University of Wisconsin-Madison have been funded by the NSF to establish the Network for Advanced NMR (NAN) to improve access to NMR resources for non-NMR experts. NAN will include ultra-high field 1.1 GHz NMR instruments at both UGA and UW-Madison and networked data portal and infrastructure through UConn. Dr. Corinne Rondeau - Mouro, INRAE of Rennes (France)
After studying organic and organo-metallic chemistry, she obtained her PhD in Chemical Sciences at the University of Rennes 1 in 1998. From 1998 to 2000, she alternated two post-doctorates confirming her specialization in the development of new methods in liquid NMR applied to structural characterization and diffusion of proteins. In 2000, she joined INRA Nantes as engineer in charge of the solid-state NMR laboratory, making a strong contribution to the study of biopolymer assemblies of plant origin (cellulose, starch). She co-founded and facilitated the BIBS (Biopolymers Interactions Biologie Structurale) platform from 2005 to 2010. In 2011, she continued her research at IRSTEA in Rennes by expanding her expertise to low-field NMR (time domain) and MRI methods for the study of hydro-thermal transformation of cereal products. Since 2020, she has been co-director of the OPAALE unit (Process optimization in agri-food, agriculture and environment) at the INRAE in Rennes while continuing her research on the NMR and MRI characterization of bioproducts with a recent focus on the role of water in the degradation of domestic and agricultural waste. She is co-author of 80 peer-review articles and book chapters with an h-index of 25 (ResearchGate) Pr. Andre Simpson, University of Scarborough Toronto (Canada)
Professor Andre Simpson co-founded the Environmental NMR Center at the University of Toronto Canada and develops nuclear magnetic resonance (NMR) spectroscopy for environmental applications. He pioneered a new technology in collaboration with Bruker Biospin, termed comprehensive multi-phase NMR, that allows all bonds in liquids, gels and solids to be detected and differentiated at the same time in unaltered biological and environmental samples. When applied to a living organism the approach allows all bonds (shell, membranes, metabolites etc.) to be detected for the first time in-vivo. His recent work developing in-vivo NMR allows detailed metabolic profiling of living organisms. This provides real-time pathway information critical to explain why chemicals are toxic and to pin-point the exact environmental stressors causing the biological perturbations. André has published more than 250 refereed articles along with 25 book chapters and 1 book. He is currently a fellow of the Royal Society of Chemistry and has been awarded a number of prestigious awards including, RSC/SETAC environmental science award, CSC Fred Beamish Award/W.A.E. McBryde Medal, the RSC Joseph Black award/medal and the 2021 RSC Horizon Award. Pr. Véronique Gilard, Toulouse University (France)
Véronique Gilard obtained a Master’s degree in physical chemistry, a PhD degree in biomolecular sciences in 1993 and her “habilitation to conduct researches” in 2006. Since 2010, she is full professor in analytical chemistry at the Paul Sabatier University in Toulouse (France). Her previous research interests were in the field of degradation and metabolism of fluorinated or phosphorated anticancer drugs followed by 31P and 19F NMR and in the analysis of brain metabolites by proton NMR. Her main current research topics are related to the development of high and low-field NMR analytical methodologies in various fields (food supplements, counterfeited drugs, illicit drugs) and to NMR-based metabolomics analysis. She was involved in the supervision of 10 PhD thesis and 30 Master students. She has authored 86 journal articles in peer-reviewed international journals and 4 chapters of book. Pr. Patrick Giraudeau, Nantes University (France)
He studied physics and chemistry at the University of Nantes, where he received his Ph.D. degree in 2008. Then he worked as a postdoctoral researcher in the Department of Chemical Physics at the Weizmann Institute of Science (Israel). In 2009, he became an associate professor at the University of Nantes, where he became a full professor in 2017, and where he now leads the analytical chemistry research group and the NMR platform. In 2014, he became a fellow of the Institut Universitaire de France, and received a consolidator grant from the European Research Council in 2019. His research activities at the CEISAM research institute focus on the development of quantitative NMR methods for the analysis of complex mixtures, including applications to metabolomics and fluxomics. Research highlights include the development of fast multi-dimensional quantitative experiments at high fields and also on benchtop spectrometers, as well as recent investigations in dissolution dynamic nuclear polarization. Prof. Patrick Giraudeau is the vice-president of the Ampere Society and of the French-speaking metabolomics society (RFMF), a member of the Euromar Board of Trustees and of the executive board of MetaboHub. He is an associate editor of Magnetic Resonance in Chemistry (Wiley) and of Magnetic Resonance (Copernicus), and a board member of JMR Open (Elsevier). He is the author of ca. 115 peer-reviewed publications. Dr. François-Xavier Theillet, Paris-Saclay University (France)
He is a CNRS researcher at the Institute of Integrative Biology of the Cell (I2BC, UMR9198 CNRS-CEA-Univ. Paris Sud). His core expertise is biochemistry and in-cell NMR. He established methods to study protein structure in cells at the atomic scale, and to investigate protein multiple chemical modifications. He focused successively on the analysis of the conformational behavior of bacterial polysaccharides and their recognition by the host immune system (PhD thesis, Institut Pasteur), and then on the analysis of the conformational behavior and the biochemistry of intrinsically disordered proteins in vitro and in cell (postdoc in Selenko lab, Berlin; and permanent position CNRS, Gif-s-Yvette). These methods found applications in studies about molecular mechanisms linked to cancers and neurodegenerative diseases. He provided the first experimental proof that the disordered state of a protein, namely a-synuclein, could be stable in mammalian cells; we also established the second ever protein structure in cells using lanthanide tags and pseudo-contact shifts. Using NMR spectroscopy, we could decipher new complex states of poly-modifications (i.e from 2 to 30 phosphorylation sites), which permits to explore poorly-described, but native forms of disordered proteins, revealing new cell signaling mechanisms and biophysical properties. He is working now on multidomain proteins integrating disordered regions and their kinases. These works rely on steady efforts in protein recombinant production and purification (using bacteria, cell-free expression, insect and mammalian cells; p53, Mdm2, Elk1, Oct4, Sox2, Esrrb, a-synuclein, …, and a dozen of kinases), and in improving NMR sensitivity (pulse sequences, novel isotopic labeling). The developed methods aim at expanding knowledge on: i) cell signaling carried out via disordered proteins and their multiple post-translational modifications; ii) structural behavior and interactions of proteins in cells. Dr. Hélène Launay, Aix-Marseille University (France)
I joined the Laboratory of Bioénergétique et Ingénieurie des Protéines (Bioenergy and protein biotechnologies) in 2018 after 10 years of experience on structural biochemistry. My doctoral thesis focused at the biosynthesis of proteins and their co-translational folding, and I am now considering the structure-function relationship of regulatory proteins in their physiological environment that is the cell. Nuclear Magnetic Resonance is one of the few biophysical tool that give access to the timescale protein conformational sampling (millisecond or faster) as well as the structural properties of intrinsically disordered proteins. Moreover, the use of selective isotopic labelling allow monitoring these structural properties in complex medium, and the use of NMR lifts the constrains of investigated proteins in isolation. I am developing now the tools to investigate biostructural NMR within living cells to decipher the regulation of the carboxylation of CO2 in the Calvin-Benson-Bassham cycle as a function of light intensity and CO2 accessibility. Pr. Leah Casabianca, Clemson University (USA)
Dr. Guilhem Pagès, INRAE of Theix (France)
Guilhem Pagés started his research career by using PFG NMR applied to chemical or biochemical systems. His PhD was awarded in 2006 in Marseille under the supervision of Prof. Caldarelli. Then, he had several post doctoral positions in this field with Prof. Kuchel in Sydney, Prof. Furó in Stockholm and Prof. Malet-Martino in Toulouse. He then moved to MRI research in Singapore before being recruited in the INRAe MRI facility AgroResonance. Guilhem focusses his research on two themes. The first one deals with developing metabolic MRI, especially the CEST contrast at high magnetic field. He used this contrast to study the metabolic evolution of chondrosarcoma, a bone cancer, in animal model or to monitor metabolite evolution in tomato during its ripening. The second one aims to bring the NMR sensor outside the laboratory, in the natural environment of the object. Currently, he is using this portable NMR sensor to characterize the hydric status of plants directly in their natural environment thanks to an ANR grant. Guilhem is leading the AgroResonance team. Dr. Isabelle Krimm CRCL Lyon (France)
Isabelle KRIMM is a CNRS researcher and currently Team leader at the Cancer Research Center in Lyon, France. The team is developing small molecules that target protein kinase CK2. The objective of the team is to generate chemical tools that can be used to decipher kinase CK2 functions in pathologies such as cancer and viral infection. The team uses diverse biophysical techniques to perform fragment-based drug design and structure-based drug design. Isabelle KRIMM has a long experience using NMR as a technique of choice to study interactions of protein targets with small molecules, in particular fragment-like compounds. She has a particular interest for the ligand-observed NMR experiments such as STD, Waterlogsy and INPHARMA experiments for the screening, the ranking and the selection of the best fragment hits that should be further optimized. She is the author of 56 publications and 2 book chapters. Dr. Cyril Charlier TBI Toulouse (France)
Cyril Charlier joined the TBI in November 2019 as a CNRS researcher in NMR team (EAD17). His main goal is to apply NMR in the framework of biotechnologies focusing on the question of catalysis ranging from the catalysis of reactions in metabolic network to the catalysis of enzymatic reactions in a bioreactor. On one hand He develops tools to maintain the spectral resolution in complex spectra and to follow such spectra as function of time to further investigate metabolism by NMR. On the other hand, He uses liquid state NMR spectroscopy to study the relationship structure-dynamics-function on enzymes. |
Prof. Casabianca’s research focuses on using Nuclear Magnetic Resonance (NMR) to examine binding between small molecules and the surface of nanoparticles. Recently, this work has focused on the plastic pollution problem, as plastic pollution that is exposed to weathering, UV light, and salt water in the ocean can break down into smaller pieces, eventually forming micro-and nano-scale plastic particles. These plastic nanoparticles have a large surface area, and can absorb toxic small molecules that are also present in polluted waterways. Understanding the interactions between these toxic small molecules and plastic nanoparticles is important in designing plastic that will be less likely to absorb small molecules as it degrades, designing regulatory policy regarding toxic small molecules allowed in fish intended for human consumption, and in using plastic for removal of toxic small molecules from waterways in remediation efforts. We have used Saturation-Transfer Difference (STD) NMR to identify small molecules that bind to nanoparticle surfaces, construct epitope maps, and understand the driving forces for nanoparticle-small molecule binding. However, solution-state NMR only gives half the picture, as it can only observe small molecules that are free in solution. Thus, we have also used comprehensive multiphase NMR to examine the solid, liquid, and gel-like phases of the same sample containing polystyrene nanoparticles and amino acids.
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